CHROMATOGRAPHY

OBJECTIVES: The objective of this experiment was to extract plant leaf pigments and find them by using the Rf values obtained from the paper chromatography technique. The system of the experiment was that every(prenominal) of the five listed pigments would be present in the extracted plant leaf according to the Rf values.

PROCEDURE/APPARATUS: The equipments utilise were a 18 x 150 mm examination organ pipe with stopper, graduated cylinders, Erlenmeyer flask, mortar and pestle, metric ruler, tall jar, acetone, trivial test tube, small brown bottle, petroleum ether, plant leaf, change parsley flakes, water, glass petri dish, stapler, sand, spatula, pipette, capillary tube, Whatman deform paper, a 50 ml chromatography solvent, and goggles (to be worn at all times). The steps to performing the experiment were:

1.         Pour 50 ml of chromatography solvent into the tall jar and cover it with half of the petri dish.

2.          take for granted ½ teaspoon of dried parsley flakes and ¼ teaspoon of sand in the mortar. Tear a plant leaf into small pieces, and daub it in the mortar along with the other materials and grind it for 10 seconds. After that, butt the ground materials in the test tube and attract sense 5 ml of acetone.

3.         Shake for 10 seconds, and subsequently 10 minutes add 5 ml of water.



Then, add 3 ml of petroleum ether and shake for 5 seconds. The pigment will eventually be found on the upper layer; remove it with a pipette and place it in a small test tube in the brown bottle. Note: dispose of the remaining materials.

4.         Draw a faint pencil disceptation on a filter paper, 1.5 cm from one edge. Then, using a capillary tube, make 6 or 7 dots on the pencil line (repeat the step till the dots are dark enough).

5.         Form the...

If you lack to get a full essay, order it on our website: Orderessay



If you want to get a full information about our service, visit our page: How it works.